tissue immunoflourescence rabbit monoclonal anti cd86  (Boster Bio)

Journal: Molecular Biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Effect of FGF2 deficiency on BMDM apoptosis and polarization. a – c FGF2 deletion increased BMDM apoptosis. a Apoptosis in BMDM deprived of FBS for 24 h was assessed by flow cytometry ( n = 4). b - c Percentage of PI + Annexin V + and PI- Annexin V + BMDM after starvation. d - k FGF2 deletion in BMDM promoted M1 polarization. d - g Flow cytometric analysis of macrophage markers in BMDM treated with LPS or IL4, including CD86, iNOS, CD206, and Arg1 ( n = 3). h - k The levels of CD86, iNOS, CD206 and Arg1 in BMDM after treatment with LPS or IL4. N represents no treatment; * p < 0.05, vs. WT; Ψ p < 0.05, vs. N + WT; Ω p < 0.05, vs. N + FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Flow Cytometry

Journal: Molecular Biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Mice reconstituted with FGF2 KO macrophages and subjected to CLP demonstrate increased M1 polarization in lung tissue. a - f The presence and levels of CD206, CD86, and F4/80 markers on macrophages within lung tissue were identified and quantitatively assessed using immunofluorescence staining. Bar is 20 μm. * p < 0.05, vs. WT; Δ p < 0.05 vs. WT + LPS; # p < 0.05 vs. FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining

Journal: Parasites & Vectors

Article Title: Role of cuproptosis in mediating the severity of experimental malaria-associated acute lung injury/acute respiratory distress syndrome

doi: 10.1186/s13071-024-06520-1

Figure Lengend Snippet: The effect of DSF or TTM on pulmonary macrophage M1/M2 polarization in experimental MA-ALI/ARDS mice. A – C Representative images of positively stained pulmonary CD68 + , CD86 + , and CD206 + macrophages in different groups using immunohistochemical staining. The positively stained CD68 + , CD86 + , and CD206 + macrophages showed a dark brown color (arrows). a Naïve mice; b P. berghei ANKA-infected control mice at 8 dpi; c P. berghei ANKA-infected control mice at 15 dpi; d DSF-treated uninfected mice; e DSF-treated P. berghei ANKA-infected mice at 8 dpi; f DSF-treated P. berghei ANKA-infected mice at 15 dpi; g TTM-treated uninfected mice; h TTM-treated P. berghei ANKA-infected mice at 8 dpi; i TTM-treated P. berghei ANKA-infected mice at 15 dpi. D The numbers of positively stained CD68 + , CD86 + , and CD206 + macrophages were calculated from more than 20 fields per animal. Differences in the numbers of positively stained cells between two groups or among multiple groups were compared using the independent-samples t -test or one-way ANOVA, respectively. Experiments were conducted with six mice per group, and data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 vs. the P. berghei ANKA-infected control mice at 8 dpi; # P < 0.05 and ## P < 0.01 vs. the P. berghei ANKA-infected control mice at 15 dpi. NS = non-significant, P > 0.05, relative to naïve mice

Article Snippet: The slices were incubated with primary antibodies [monoclonal mouse anti-SLC31A1 antibody (1:200; no. NBP2-36573; Novus Biologicals), monoclonal mouse anti-ATP7A antibody (1:100; no. MA5-27720; Thermo Fisher Scientific), polyclonal rabbit anti-FDX1 antibody (1:200; no. 12592-1-AP; Proteintech), monoclonal rabbit anti-CD68 antibody (1:200; no. 97778S; CST), monoclonal rabbit anti-CD86 antibody (1:200; no. 19589S; CST), or polyclonal rabbit anti-CD206 antibody (1:400; no. GB113497-100; Servicebio)] overnight at 4 °C.

Techniques: Staining, Immunohistochemical staining, Infection, Control

Journal: Parasites & Vectors

Article Title: Role of cuproptosis in mediating the severity of experimental malaria-associated acute lung injury/acute respiratory distress syndrome

doi: 10.1186/s13071-024-06520-1

Figure Lengend Snippet: The effect of co-culture of DSF-CuCl 2 or TTM-CuCl 2 on the mRNA levels of SLC31A1, ATP7A, FDX1, CD86, CD206, TNF-α, iNOS, TGF-β, and IL-10 in iRBC-stimulated RAW 264.7 cells in vitro. The RAW 264.7 cells were pretreated with 5.0 × 10 6 iRBCs for 6 h, then treated with PBS, DSF-CuCl 2 , or TTM-CuCl 2 for 24 or 48 h. The qPCR method was used to quantify the mRNA levels of target genes. & P < 0.05 and && P < 0.01 vs. RAW 264.7 cells treated with only PBS for 24 h; * P < 0.05 and ** P < 0.01 vs. iRBC-stimulated RAW 264.7 cells for 24 h; $ P < 0.05 and $$ P < 0.01 vs. RAW 264.7 cells treated with only PBS for 48 h; # P < 0.05 and ## P < 0.01 vs. iRBC-stimulated RAW 264.7 cells for 48 h. Data are taken as mean ± SD. The experiment was conducted 4–5 times with similar results

Article Snippet: The slices were incubated with primary antibodies [monoclonal mouse anti-SLC31A1 antibody (1:200; no. NBP2-36573; Novus Biologicals), monoclonal mouse anti-ATP7A antibody (1:100; no. MA5-27720; Thermo Fisher Scientific), polyclonal rabbit anti-FDX1 antibody (1:200; no. 12592-1-AP; Proteintech), monoclonal rabbit anti-CD68 antibody (1:200; no. 97778S; CST), monoclonal rabbit anti-CD86 antibody (1:200; no. 19589S; CST), or polyclonal rabbit anti-CD206 antibody (1:400; no. GB113497-100; Servicebio)] overnight at 4 °C.

Techniques: Co-Culture Assay, In Vitro